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        <title>LRE Literature</title>
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        <h1 align="center">LRE Literature</h1>
		<p>Rutledge RG, Stewart D (2008a) A kinetic-based sigmoidal model for the 
		polymerase chain reaction and its application to high-capacity absolute 
		quantitative real-time PCR. BMC Biotechnol 8: 47</p>
		<p>Rutledge RG, Stewart D (2008b) Critical evaluation of methods used to 
		determine amplification efficiency refutes the exponential character of 
		real-time PCR. BMC Mol Biol 9: 96.<br>
		<br>
		Rutledge RG, Stewart D (2010) Assessing the performance capabilities of 
		LRE-based assays for absolute quantitative real-time PCR. PLoS ONE 5: 
		e9731.<br>
		<br>
		Rutledge RG, Stewart D (2011) A Java program for LRE-based real-time 
		qPCR that enables large-scale absolute quantification. PLoS ONE 6(3): 
		e17636</p>
		<p><font color="#0000FF">Two early studies that were instrumental in the 
		development of LRE qPCR:<br>
		</font>Rutledge RG, Côté C (2003) Mathematics of quantitative kinetic PCR 
		and the application of standard curves. Nucleic Acids Res 31(16):e93</p>
		<p><a name="SCF"></a>Rutledge RG (2004) Sigmoidal curve-fitting 
		redefines quantitative real-time PCR with the prospective of developing 
		automated high-throughput applications. Nucleic Acids Res 32(22):e178</p>
		<p><font color="#0000FF">An example of gene expression profiling using 
		LRE-based absolute qPCR that included analysis of nine reference genes:<br>
		</font>Rutledge RG, Stewart D, Caron S, Overton C, Boyle B, MacKay J, 
		Klimaszewska K (2013) Potential link between biotic defense activation 
		and recalcitrance to induction of somatic embryogenesis in shoot 
		primordia from adult trees of white spruce (Picea glauca). BMC Plant 
		Biology 13, 116. </p>
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